The step one plus RT-PCR ( 96 well platform, 4 color system, veriflex sample blocks) use fluorescent-based PCR reagents to provide 1) Quantitative detection of target nucleic acid sequences using real time analysis, 2) Qualitative detection of targets using post-PCR (end point) analsyis, 3) Qualitative analysis of the PCR product (achieved by melt curve analysis that occur post-PCR) and 4) Time saving ( one full run of PCR plate completed within 40minutes ). The step one plus RT-RCR instrument contains 6 independently thermally regulated "veriflex" blocks to help to optimize the thermal cycling conditions. We can set a different temperature for one or more of the veriflex blocks, (or) we can set the same temperature for each of the veriflex blocks. The following experiments can be perform using the step one plus RT-PCR:
- Quantitation - Standard curve
- Quantitation - Relative standard curve
- Quantitation - Comparative CT
- Melt curve
- Genotyping
- Presence / Absence experiments
- Minimizing DNA contaminants
Following 4 filters can be used by the step one plus RT-PCR : 1) FAM dye - SYBR Green dye, 2) JOE dye - VIC dye, 3) TAMRA dye- NED dye, and 4) ROX dye.
